Typhoid fever is an acute infectious disease caused by Salmonella typhi, is characterized by fever, dirty tongue, abdominal pain, diarrhea, vomiting, headaches, seizures and impaired consciousness. The purpose of this study was to determine the relationship between antibody titer results with the presence of Salmonella typhi z66Ind gene in patients with typhoid fever in the city of Jayapura, Papua province of Indonesia. This study uses laboratory exploration studies with a total sample of 92. The test is used to detect the presence of Salmonella typhi z66Ind genes that widal test, lateral flow test, culture test and analysis of PCR amplification. The results showed that discovered the gene z66Ind Salmonella typhi in the Widal test titre Ag O 1/320 and Ag H 1/320, lateral flow test (2+), a culture test (+) Salmonella typhi and positive PCR results (+) on electrophoresis will formed 0.6 kb fragment of DNA bands in patients with typhoid fever in the city of Jayapura.