Pleurotus sajor-caju, a nutrient rich mushroom was cultivated on four different substrates, viz. Paddy straw, Wheat straw, Apple leaf and Chinar leaf substrates. It was observed that its yield or biological efficiency was maximum on Paddy straw followed by Wheat straw, Apple leaves and Chinar leaves. It was also observed that P. sajor-caju gave the maximum yield in the first flush followed by second, third and fourth flush except in case of Chinar leaves where the yield obtained in second flush was comparatively higher than first flush.