In an earlier study, we isolated α-mangostin as the major xanthone and 3-isomangostin as a minor constituent of the pulp of the ripe fruit of Garcinia mangostana Linn. Further study on the pulp has led to the isolation of garcinone D (1). The structure of 1 was elucidated by extensive 1D and 2D NMR spectroscopy. Garcinone D was reported to exhibit anticancer property.