To evaluate some antioxidant enzymes in 11 wheat cultivars from Khuzestan province, Iran, this experiment was conducted in 2011. Experimental design was randomized complete block design with four replications, and the evaluated factors were alpha-amylase, catalase and glutathione peroxidase. Results indicated that alpha-amylase content was significantly the same in the 11 cultivars; however, catalase and glutathione peroxidase content varied significantly between the cultivars (P≤0.01). Mean comparison indicated that the highest catalase and glutathione peroxidase content (1.32 mM/min/g fresh weight and 0.079 mM/min/g fresh weight, respectively) were related to Chamran cultivar. Generally, results of this experiment showed that Chamran, S 78, Karkheh, Dena, Behrang and Veryanak contained the highest catalase and glutathione peroxidase.