The modern methods of analysis are essential for qualitative and quantitative determination of herbal formulations and standardization as per the WHO guidelines. In present study simple and precise UV spectroscopic method was developed and validated as per ICH guidelines for the estimation of the active constituents in the polyherbal Ayurvedic. The method was developed using methanol: water (50:50) mixture as solvent. The recovery studies were performed by standard addition method. The method was found to be validated and can be used for routine analysis of herbal formulation containing withanolide, colchicine and gingerol.
