A novel, simple, rapid and precise reverse phase isocratic high performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous estimation of Atorvastatin and Ezetimibe in pure and marketed formulations. Estimation of drugs in their formulations were done with a Kromasil 100-5C18 Column[ 250mm × 4.6mm]using mobile phase of composition Methanol and Water (80:20 v/v)the flow rate was 1ml/min and the effluent was monitored at 257nm. The retention time of Atorvastatin was observed at 2.1min.The retention time of Ezetimibe was found to be 4.8min.The method was found to be linear over a range of 10-50mg/ml for both Atorvastatin and Ezetimibe. The method was validated according to the guidelines of International Conference on Harmonization (ICH) and was successfully applied in the estimation of commercial formulations.
