A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Atorvastatin and Atenolol in tablets. The compounds were separated on an ODS analytical column with a mixture of Acetonitrile, and Phosphate buffer (pH 4.5±0.05 adjusted with ortho phosphoric acid) in the ratio 72:28 (v/v) as mobile phase at a flow rate of 1.0 mL min-1 at 238 nm. The retention times of Atorvastatin and Atenolol was found to be 2.167 and 3.737 minutes. Calibration plots were linear over the concentration ranges of 4–20 μg mL-1 and 20–100 μg mL-1 respectively. Validation studies revealed the method is specific, rapid, reliable and reproducible. Stability or stress studies was carried out for acidic alkaline, oxidative hydrolysis, thermolytic, relative humidity and photolytic exposure on the drug substance and drug product The high recovery and low relative standard deviation confirm the suitability of the method for determination of atorvastatin and atenolol in tablets.
