The objective of the study was to develop a simple, accurate, precise and rapid a UV spectrophotometric i.e. first and second order derivative methods for the determination of thiocolchicoside in pharmaceutical dosage form i.e. capsules by using methanol as a solvent. The method was further validated by ICH guidelines. The proposed derivative methods involve the measurement of absorbance at 251.8 nm for first order and 224 nm for second derivative for the estimation of thiocolchicoside respectively. The linearity of the proposed method was found in the concentration range of 1 to 14 μg /ml (r2= 0.9993) for first order derivative and 1 to 14 μg /ml (r2= 0.9987) for second order derivative methods respectively. The percentage mean recovery was found to be 100.365 % for first order derivative and 100.31% for second order derivatives methods respectively. The methods were also statistically validated for its linearity, accuracy and precision. Both intra and inter day variations showed less percentage (%) RSD values indicating high grade of precision of this methods.
