Simple and precise UV spectrophotometric methods by first order derivative and area under curve [AUC] - have been developed and validated for the estimation of rupatadine fumarate in bulk and its tablet formulation. The standard and sample solutions of rupatadine fumarate were prepared in absolute alcohol. Rupatadine fumarate was estimated at 214 nm for the first order derivative UV-spectrophotometric method (A), while in area under curve (AUC) method (B) the zero order spectrum of rupatadine fumarate was measured in between 245 nm to 255 nm. Beer’s law was obeyed in the concentration range of 1 to 30 μg / ml with coefficient of correlation value 0.9998 for first order derivative method. Similarly in AUC method, Beer’s law was obeyed in the concentration range of 1 to 30 μg / ml with coefficient of correlation value 0.9988. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation were of 2.949 % and 1.9267 % for the above two methods respectively. The proposed methods were successfully applied for the determination of rupatadine fumarate in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments.
