The growing demands on the steroids encouraged searching for new sources. Jatropha sp. is rich sterols. The common sterols included β-sitosterols, campsterol and stigmasterol. An isolated strain of Absidia corymbifera showeda potentiality to transform some sterols to important steroids, Androsteindione (AD) and Androstadienedione (ADD) as a major products. These are medically important in steroids. An optimization depends on two levels factorial design as a screening forthe effective factors from seven cultural factors and three levels model for optimization of the effective factors were used. The most significant factors found to be the molasses concentration, time and temperature. These factors were further optimized using the three levels factorial design depending on polynomial quadratic equation. The products yields were 32 % for ADD and 15 % for AD at the beginning of the optimization process. After the statistical optimization the yields reached 77 % for ADD and about 18% for AD.
