The aim of the present study was to measure the biomarkers in blood serum of round goby Neogobius melanostomus and scorpion fish Scorpaena porcus (L.) caught in two bays of the Black Sea in Sevastopol region (Crimea) which were differed of pollution level. Concentration of oxidated proteins (OP) was studies in blood serum spectrophotometrically at 345, 370, 430 and 530 nm. The OP level in blood serum of fish from polluted area was higher than in less-polluted site. The interspecies differences of OP concentration in two tested fish species were shown and depended on specific features of biology and ecology. High anthropogenic impact leads oxidative stress in fish tissues and stimulates high production of ROS, which damage and modify cell membranes and biomolecules, including proteins. Examined biochemical parameters are important for the evaluating of fish abilities to protect against chemical pollution and keep their life in the polluted environments. They may potentially be used as indicators of chemical pollution of marine environment and fish health status.