The bioactive ethyl acetate and n butanol soluble parts of successive methanol extract of fruits of Lagenaria siceraria were subjected to column chromatography over silica gel (60-120 mesh) for separation of different phytoconstituents. Two flavonoids, a triterpenoid and a mixture of sterols were isolated. The structures I-V, were obtained through spectral analyses as oleanolic acid, β sitosterol, campesterol, isoquercitrin (quercetin-3-O-β-D-glucose) and kaempferol respectively. Their structures were elucidated by means of UV, IR, mass, and NMR spectroscopic analyses. These compounds were identified by comparison of their 1H-NMR, 13C-NMR and mass spectra with the literature. The isolation of oleanolic acid, kaempferol and isoquercitrin from Lagenaria siceraria fruits is being reported first time. These compounds may explain the medicinal value of fruits of Lagenaria siceraria.
