The main aim of the present research work is to evaluate the safety of the traditional siddha medicine Natural PooraParpam (NPP) and Synthetic PooraParpam (SPP) by determining its possible toxicity after acute and subacute administration in rodents. In short term acute toxicity study the drug NPP and SPP was administered in single doss of 1.28 mg / kg b.w (p.o). Potential drug toxicity related to C.N.S, A.N.S and C.V.S were observed up to 14 days. In sub-acute toxicity study the drug NPP and SPP was administered at three dose level such as low dose, middle dose and high dose of 1.15, 1.44 and 2.88 mg / kg b.w (p.o) for four weeks, 5ml of KarumbuVellam (Cane sugar)] mixed with water served as vehicle control group and saline treated group served as normal control. Results obtained from the acute and sub-acute study reveals that the drug NPP and SPP doesn’t cause any significant change in behavior, mortality and other potential adverse effects on treated animals. Throughout the study period no sign of toxicity was registered. Further it was observed that NPP and SPP at all three dose level did not modify the weight index, food and water intake in treated animals. There is no significant change in hematological, biochemical and histopathological observation of animals treated with NPP and SPP at the dose of 1.15, 1.44 and 2.88 mg / kg b.w (p.o) when compare to control group animals. From the result it was concluded that both the formulation NPP and SPP offers wide margin of safety in tested rodents and further long tern usage of drugs will be considered as safe for the ailment of various disease.
