Cisplatin is widely used as an antitumor drug. To reduce its toxic side effects in patients .Cisplatin is a chemotherapy drug. It is used to treat various types of cancers, including sarcomas, some carcinomas , lymphomas, and germ cell tumors. It was the first member of a class of platinum-containing anti-cancer drugs, which now also includes carboplatin and oxaliplatin. These platinum complexes react in vivo, binding to and causing crosslinking of DNA, which ultimately triggers apoptosis. Since cisplatin provokes fertility and induces germ cell apoptosis and necrosis Therefore, we have been tried in this study to evaluate the toxic effects of this drug on Rat testis tissue. In this study we choose 40 adult male Rat. The Rats were divided randomly into 2 groups as Control and Experimental. Rats of the experimental group were injected by Cisplatin as 3 dose (20mg/m2/5days- IP) .The Control group injected by normal Salina. At the termination of experiments the testis were fixed and stained with H&E and Trichrome Masson. Data were analyzed by T-Test and SPSS software.Microscopic study shows changes includes: The diameter of seminiferous tubules and epithelial thickness and the average percentage of tubules with spermatozoa was significant diminished. The spermatogenic cells were significant decreased with degenerative changes. Interstitial testicular edema probably occurred in cisplatin-treated rats .In comparing the average of the parameters in the experimental group with control group, there was significant difference (P<0.001). The study showed that cisplatin-induced germ cell apoptosis in the Rat testes. This study suggests that Cisplatin impairs spermatogenesis and testis function. It is suggested that cisplatin-induced germ cell apoptosis may result in decreased spermatogenesis. These finding will have an important bearing for young receiving.
