Santolina africana Jord. Fourr is of the Asteraceae family, an endemic plant that grows in northeastern Algeria, which has great benefits in traditional medicine (used as antiperspirants, antiseptics, antiseptics, bactericidal and anti-diabetic). [1] The studies have demonstrated antioxidant activity and bacteria for volatile oils of this plant [2]. Firstly, Preliminary tests for chemical families have been shown presence of (flavonoids, tannins, coumarins, alkaloids, and sugars). The content of phenols, flavonoids was estimated by spectrophotometric methods. Also the antioxidant activities of the extracts of S. africana were investigated by DPPH test and phosphomolybdenum assay. The results of the quantitative estimation of total phenols by Folin-Ciocalteu reactif showed that the n-butanol phase had the highest ratio (123,3875 mg GAE /g). The results of the quantitative evaluation of flavonoids by trichlorure d 'aluminum showed that the phase of the petroleum ether contained an excess of flavonoids in the unusual results for the test anti-oxidant of test the DPPH . The n-butanol phase showed the best result followed by the acetate phase (IC50=45.90 ± 0.573 & 38.80 ± 1.201 mM respectively). The result of TAC test the n-butanol extract gave the highest inhibitory (TAC=1.227 ± 0.02 (mM)) concentration followed by the petroleum ether, acetate and, chloroform phase. Antibacterial activity was performed on two Gram negative strains (Escherichia coli ATCC25921, Pseudomonas aeurginosa ATCC29733), and a third Gram-
