To determine the seroprevalence of Human Immunodeficiency Virus in pregnant women in a tertiary care hospital, South India. This study was conducted at Shadan Institute of Medical Sciences and PostGraduate Research Centre, Hyderabad, during August – 2007 to May 2012 . The study included 4384 antenatal women, all of whom were screened for HIV. of the 4384 antenatal women, 12 were found to be positive for HIV (0.27%)