To study the sensitivity pattern of Escherichia coli to Levofloxacin in a tertiary care hospital. The study was done for a period of 2 years from January 2010 to December 2011. A total number of 945 patients were included in the study. Cases which were culture positive for Escherichia coli were identified and their sensitivity pattern for Levofloxacin was assessed. 81 patients were found to be having Escherichia coli infection . Among these 81 patients having Escherichia coli infection 66 patients (81.4%) were sensitive to Levofloxacin.