Overtraining syndrome is a chronic situation that results from long periods of high intensity or high volume work without rest periods. The purpose of this study was to determine the relationship between plasma testosterone and cortisol concentrations with psychological overtraining items (by Consensus group on overtraining of the Society Francoise de Medicine due Sport) in football referees. Thirty football referees (X-±SD: 26.1±3.19 y; 182±4.21 cm; 72.4±6.61 kg; 23.97±1.72kg/m2; 51.21±2.53 Vo2max) volunteered for the study during a rest day (24 hours without training). They were asked to complete the overtraining questionnaire contains 54 question requiring answers of \"yes\" or \"no\". Then plasma samples were taken at rest and immediately after a game of Isfahan premier league(Asia vision).The results of data analyses showed that the overtraining score from questionnaire correlates with cortisol concentration on rest day respectively (r=0.71), and testosterone/cortisol ratio (r=-0.42; p£0.05). Result show that, the questionnaire may be a useful tool for monitoring and preventing of overtraining syndrome.
