The main aim of this research is study the relationship between football referees’ hormonal status and their Performance. The population is the working professional referees in super league and first league of Iranian football. They are 90 (N=90) people who work in 2012-2013 season and 30 (n=30) of them are sampled randomly for the study. referees’ saliva was sampled at two levels: first, before starting the match, finally immediately after the match. The sampling was done for three matches for per referee and the samples were sent to the lab for analyzing. The calculations were done using Pearson's correlation coefficient and Statistical Package for the Social Sciences (SPSS Inc., version 18.0) was used for the analyses. P-values below 0.05 were considered statistically significant. Results showed that there is a significant negative correlation between referees’ hormonal status and performance. That is the referees’ hormonal status had an impact on how they judged.