The essential oil obtained from the aerial parts Leonurus cardiaca L. subsp. persicus (Boiss.) Rech. F., endemic to Iran, was analyzed by using GC and GC-MS. L. cardiaca subsp. persicus with a yield of 0.25% (w/w), consist of forty six compounds representing 90.03 % of the essential oil. The major components in the latter one included Thymol (35.25%), Germacrene D (7.62%), Borneol (6.69%), trans-Caryophyllene (4.83%), γ-Guaiol acetate (4.43%), Phytol (4.10%) and β-Phellandrene (2.85%). The differences between the populations of the current study and the previous studied ones may be because of the time of collecting the specimens, chemotypes, drying conditions, mode of distillations, geographical and climatic factors.