Replication-defective retroviral vectors are currently the most efficient gene transfer method for the production of transgenic animals. Transgenic animals have become important tools for biological research. In vertebrates, foreign DNA is routinely introduced into the genome by microinjection into newly fertilized zygotes. . Necrosis virus-based SNTZ vector carrying the E. coli lacZ gene designed to express a nuclear-localized β-galactosidase was injected into the genome. In this study, performance of the SNTZ vector carrying the E. coli lacZ gene to express a nuclear-localized β-galactosidase was injected into the subgerminal cavity of embryos collected from newly laid eggs will be reviewed.
