European Journal of Zoological Research
Abstract
Author(s): Hamed Amini Pour, Abdol-Mansour Tahmasbi and Abbas-Ali Naserain
The aim of this study was investigation of various levels of vitamin E in Tris extender on semen characteristics of
Ghezel rams in pre and post freezing conditions. Semen was collected from six healthy and mature rams with
average 55Ã?â??Ã?±5 Kg body weight by electro ejaculator. High quality samples were mixed and diluted in Tris extender
after apply different concentration of vitamin E. Treatments were various levels of vitamin E (0, 1, 2, 3, 4 and 5
m/M). The semen aliquots were chilled and preserved at 5Ã?â??Ã?°C and their quality was evaluated in pre freezing, and
then cooling semen samples entered into 0.5 ml of straws. At first, straws were frozen upper vapor liquid nitrogen,
and then kept in -196Ã?â??Ã?ºC. After 10 days straws were thawed and some characteristics of spermatozoa were examined.
Analysis of data was carried based on completely randomized design. Results of this study showed that the effect of
vitamin E on characteristics of viability, motility, progressive motility and normal of spermatozoa were significant
in pre and post freezing conditions (P<0.05), and the highest percentages of this treats were obtained in treatment
of 2m/M vitamin E. In conclusion for storage long term of Ghezel rams spermatozoa, using 2mg/ml of vitamin E in
semen extender is recommend.
