The effects of Nigella Sativa seedsof medicinal plants on performance, carcass characteristics and humoralimmunity of broiler chickens were studied in this experiment.At first 225 one day old broiler chicks were divided to5 groups and 3 replicates of 15 chicks in each group. Experimental groups included T1, control group with no Nigella Sativaseedssupplementation, T2,T3, T4,and T5 received 0.75% ,1%, 1.5%, and 2% Nigella Sativarespectively. The results showed that the use of different levels of Nigella Sativaseedshas significant effects on performance and carcass traits of broilers (P<0.05). The highest level of weight gain was in group 5 alsothe highest percent of liver and breastwere observed in group5 but the greatest percent of thigh was observed in group 4. The results evidence that the using of Nigella Sativa seedsin broiler feeds have significantly effects on performance and blood biochemical and carcass traitswithout having any significantly effects on immunity parameters except the level of heterophile to lymphocyte.