The aim of this study was to investigation the effects of in ovo injection of glucose on characters of hatching and parameters of blood in broiler chickens. 576 fertile eggs were divided to four groups; 1) control group (without injection), 2) group including 0.5 ml deionized water (sham group), 3) group including 0.5 ml glucose 20% in deionized water, 4) group including 0.5 ml glucose 25% in deionized water with four replicates per treatment and 36 eggs per replicate. After hatching, characters of hatching and concentration of blood’s glucose, triglyceride and cholesterol were determined. Results showed that control group had significantly higher percent of hatching than other groups, but group glucose 20% and 25% as compared with control group and sham group had significantly higher the weight of newly-hatched chickens (p<0.01). Amount of blood’s glucose on the first, 21st and 42nd had significant difference in group glucose 20% and 25% than other groups (p<0.01). In 21 day, group including 0.5 ml glucose 20% had higher amount of blood’s triglyceride than other groups (p<0.01), but amount of blood’s cholesterol hadn’t significant difference in broilers. In 42 day, amount of triglyceride and cholesterol hadn’t significant difference in broilers. Data Suggest, in ovo injection can be reduced hatchability and increase weight in newly-hatched chickens.
