Queen bee larva needs nutrients such as proteins, carbohydrates, minerals, vitamins and water to provide its energy, growth, and development. These materials are provided by royal jelly.The jelly is produced by worker bees and poured in queen cell. This study was conducted to produce queens with desired physiological specifics and high reproduction ability. 0.02, 0.04, and 0.06 mg of oleic acid were mixed with royal jelly and added to queen cell in five days after grafting. Twelve days after grafting queens were born and birthweightwas measured. Five days after queens spawning, ovary weight and the number ofoocytes were measured. The spawning amount was measured in two stages: 20 days after spawning and 60 days after spawning. According to results, oleic acid levels affected birth weight, ovary weight, the number of oocytes in each ovary and spawning amount which were increased 41.1, 59, 29.3, and 57.4%, respectively (p<0.05) in proportion to control group. Significant differences were observed also between the groups in second spawning. Considering the results,adding oleic acid affected reproduction parameters and will increase reproduction ability of queen.
