Although Fe is easily found in earth crust and soil, it is not available to plants in most soils especially in calcareous soils of dry warm climates with high pH. So this study was conducted in 2010 to evaluate the effect of Thiobacillus and Pseudomonas fluorescent inoculation on maize growth and Fe uptake in a calcareous soil. The experimental design was factorial in the form of a completely randomized, with four replications. Treatments of the experiment were sulfur (0 and 100 kg/ha), P. fluorescent (with and without inoculation) and Thiobacillus (with and without inoculation). Five maize seeds of S.C. 704 cultivar were planted in each pot containing 7 kg soil, after germination two of them were thinned to reach the density of three plants/pot. During the 90 days of growth period, soil moisture was held at 70% of field capacity. Before the final harvest, leaves chlorophyll content were measured. Plant's fresh and dry weight, plant height and Fe uptake were also measured after harvest. Results indicated that Thiobacillus had no significant effect on any of the measured traits; sulfur and P. fluorescent significantly affected the measured traits (P≤0.05). Maize Fe uptake was also affected by P. fluorescent inoculation.