In order to study the effect of pre treatment of wheat seed (Triticum aestivum L.) by pyridoxine on germination and development characteristics of its herb as factorial in the frame of the plan was completely three times accidentally. Factors include pyridoxine with densities (witness), 0/02, 0/07, 0/06 % and its duration application was 8, 16 and 24 hours. Germinated seeds were counted daily for eight days. Then the percent of germination, germination speed, the average daily germination, the length of the shoot, root and the weight of dry plant and also the amount of catalase and peroxidase activity were measured. The results showed that pyridoxine affects on the percent of germination, germination speed, the average daily germination, the weight of dry plant, the length of the shoot and the root and the amount of catalase and peroxidase enzyme activity on the level of one percent (p>0/01) meaningfully. These results showed that duration application of the pyridoxine on the percent of germination, the weight of dry plant, the length of the shoot and the root, the amount of catalase and peroxidase enzyme activity on the level of one percent (p>0/01) have meaningful effects but it doesn’t have meaningful effect on the percent of germination and the average daily germination and in other word the effect of pyridoxine and its duration application on the all measured characteristics on the level of one percent probability (p>0/01). In sum the results showed that pre treatment seed by the pyridoxine can help the farmers in setting and producing suitable wheat plant.
