Annals of Biological Research
Abstract
Author(s): Azadeh Afrigan, Zahra Javdani, Esfandiar Jahantab, Ruhollah Jahanbin and Abdol Azim Bahari
One of the effects of drought is the reduction of germination, seedling establishment and seedling growth.
Pretreatment improves some aspects of seed germination and seedling establishment. To investigation the effect of
pretreatment with Gibberellic acid on the seed germination of Secale Montanum in stages of growth in vitro and pot
under drought stress conditions experiment was conducted in laboratory and greenhouse Tehran University.
Experiments design in factorial randomized complete block with three replications. Treatments examination
included two levels of priming at 10 and 15 Ã?â??Ã?°C (first factor), Gibberellic acid treatments on three levels: 50, 100 and
150 ppm for 12 h (second factor) and osmotic potential (PEG 6000) in three 10 -, 12 - and 14 - times (factor III),
respectively. The results showed that pretreatment with Gibberellic acid of seed in drought stress conditions in vitro
increased the germination percentage, germination rate, root length and shoot length and also in medium pot,
seedling establishment percentage, seedling establishment rate, root length and shoot length as influenced by
different hormonal treatments, with Gibberellic acid 50 ppm at 10 Ã?â??Ã?°C and Gibberellic acid 100 ppm at 10 Ã?â??Ã?°C and -
10 times surface in the experimental vitro and pot have had the most positive effect on germination and growth
factors, respectively. The seed priming treatment at 10 Ã?â??Ã?°C compared with 15 Ã?â??Ã?°C displayed a better outcome.
