In order to study the effects of iron sulfate spraying on qualitative characteristics in three cultivars of dryland wheat, an experiment in the form of random complete block design (RCBD) as a split plot with three replications and twelve treatments was conducted at the Research Farm of Khorram Abad Islamic Azad University in the crop year 2011-2012. The main plot of the spraying treatment was at four levels of marker (without spraying), spraying with such concentrations as 2/1000, 4/000, and 6/1000, and the sub-plot of the wheat cultivar was at such three levels as Azar 2, Kouhdasht, and Zagros. Variance analysis and comparing the means was conducted for grain's characteristics, yield, yield components, protein, and gluten, by Duncan Test. Data analysis showed that spraying treatment has a significant effect on grain yield, and proves significant at 1% level. Maximum grain yield is 8125 kg/ha, belonging to the spraying treatment with a concentration of 6/1000 and Zagros 2 cultivar (m4v3), having a yield increase of 3761 kg/ha compared to the marker cultivar. Also, spraying has positive effects on grain's protein and gluten. As spraying concentration increases, wheatear weight, 1000-grain weight, and yield also increase. In addition, grain's gluten and protein percentage has increased compared to the marker treatment. It can be inferred from the results that its genetic record mainly determines the quality of plant gluten. Gluten quality cannot change due to variations in environmental conditions or fertilizer application. Moreover, high yield in any condition may not indicate more desirable protein and gluten. Higher protein level, mainly increasing the quality of bakeries, may be indicative of gluten with a good quality.
