Creatine is among the main ergogenic agents used in sports aiming to achieve increased power, performance, lean body mass (LBM) and delayed fatigue. Creatine supplementation has been associated with increased LBM and strength and reduced muscle mass loss.The aim of this study was the effect of creatine supplementation on fat free mass in handball players.24 handball players were randomly assigned in a double-blind method to either a creatine treatment (CT, n=12) group or a placebo (PL, n=12) group. The CT group ingested 20 g daily (5 g × 4 times) for five days. The effect of creatine supplementation was tested usingpaired t test and independent t tests for comparisons data. Statistical significance was set at p<0.05.The test results showed that creatine supplementation had significant effect on fat free mass of handball players included in groups CT. fat free mass was significantly higher than placebo group (p<0.01). There was no significant change in placebo group between pre- and post-test in fat free. on the other hand body weight in CT group significant change about 0/6Kg (p<0.01). Independence t test results also showed that the mean difference between groups in pretest and posttest was not statistically significant.This study suggests that short-term creatine supplementation significantly changed handball player’s fat free mass.