Artemisia annua L is an aromatic annual herb endemic to the northern parts of Chahar and Suiyuan provinces in China. In order to consider impact of biological and chemical fertilizers (N, P) on protein content that exist in Artemisia annua L. an experiment was carried out in factorial design in completely randomized design with 4 replications in Zabol University in 2011. Treatments included chemical fertilizers (N, P) in 4 levels (N0P0, N40P40, N80P40, N80P80) and biological fertilizers in 4 levels (control, Nitroxin [include bacteria which stimulus growth (Azotobacter and Azospirillum)], Bio-phosphorus [(include bacteria which stimulus growth (Bacillus and Pseudomonas)] and Vemricompost fertilizer. About protein content, the result showed that applying biological fertilizers specially Vermicompost has better effect than chemical fertilizers, also Vermicompost + N80P80 had better impact on protein content in comparison with other treatments.