This experiment was carried out to evaluation usage different levels of additional choline supplement (0, 400 and 800 mg/kg) and its effect on performance and visceral organs weight of Japanese quails in corn and Iranian soybean meal based diet. In this one, it was used of 240 one-day quails. This one was designed based on completely randomized designed in 3 treatments, 4 replicates and in every one 20 pieces was designed. Experimental treatments were: 1) basal diet (control), 2) basal diet + 400 mg/kg of choline supplement and 3) basal diet + 800 mg/kg of choline supplement. Time length of breeding was 42 days. Under consideration factors including performance factors (weight gain, food consumption, food conversion ratio) in total 1-42-day period and visceral organs weight (heart, pancreas, spleen, carcass and liver) which has been calculated as percentage of live body weight for both sexes, were analyzed. Results showed that addition of additional choline supplement into corn-soybean meal based diet had no effect on chick's performance in full period, also results showed that addition of various levels of choline to diet based on corn and soybean meal had no meaningful effect on visceral organs weight compared to control group, but the sexual effect on liver, pancreas, heart weight (p<0.01) and carcass weight (p<0.05) was meaningful which shows the difference between two sexes in these cases.