The purpose of this study was to examine the effect of active learning on academic achievement motivation in high schools. Participants included 1013 students that studied in Karaj high schools. There were 561 boys and 462 girls, and their ages ranged from 15-18 years-old. To data collection, all subjects filled in the Achievement Motive Scale Test (AMST) and demographic questionnaire. Also, the collected data was analyzed by inferential statistical tests such as a independent t test at the P<0.05 significant level. Results showed that the differences between two groups were significant at the level of P<0.05 and active learning group obtained higher scores than traditional group in achievement motivation. Based on our results, the use of active learning method in classroom is vital to have a positive impact on the quality of the students learning process and achievement motivation.