Plants are the essential and integral part in complementary and alternative medicine. Metabolite secondary in plant can be use as alternative medicine and heal some disease. The jackfruit (Artocarpus heterophyllus Lam) has been used as traditional folk medicine for asthma, wound healing, ear ache, antiulcer, adsorbent, antibacterial, antiinflammatory, anemia, dermatitis, cough, diarrhea, and fever. This research aimed to study the activity from methanol extract of jackfruit leaves (MEJL) in treating rheumatoid arthritis. The active compounds of jackfruit were extracted by maceration using methanol. This study begins with an acute anti-inflammatory test followed by antirheumatoid arthritis in rat model. In the acute anti-inflammatory test, Wistar rats were induced having acute inflammation by injecting intradermal of carrageenan lambda into hind limb footpad. Animal model of rheumatoid arthritis (RA) were performed in Wistar rat by injecting collagen type II bovine emulsified in CFA for immunization phase and in the IFA as booster phase. Two doses of extracts were tested i.e., 150 and 200 mg/kg body weight for MEJL. The parameters were observed i.e., arthritis score, TNF-α level and histology of joint. In the AR model test, both doses of MEJL can reduce arthritis scores significantly (p<0.05) with the highest percentage of reduction i.e.41.02% occurred after administered of MEJL at dose of 150 mg/kg body weight. MEJL at dose of 150 mg/kg body weight can reduce level of TNF-α significantly (p<0.05) compared than control. Through histological examination of hind limb joints of RA rats showed an improvement of the joint cartilage indicated by the absence of pannus formation and cartilage erosion after treated with MEJL dose of 150 mg/kg body weight. It can be concluded that MEJL dose of 150 mg/kg body weight have a better activity in treating rheumatoid arthritis compare to MEJL dose of 200 mg/kg body weight.
