Green synthesis of silver nanoparticles using cane molasses was investigated and the effect of broth concentration in reduction mechanism and particle size is reported. Cane molasses was chosen as eco-friendly and stabilizing agent replacing chemicals that pose a biological risk to the environment. The reduction of silver (Ag+) ions by cane molasses was characterized using UV-Visible spectroscopy and Transmission electron microscopy. UV-Visible spectra showed peak at 440nm. Transmission electron microscopy (TEM) showed silver nanoparticles in the size range of 10-50nm and are spherical in shape. The antimicrobial activity of silver nanoparticles against Bacillus subtilus, E. coli and Staphylococcus aureus, revealed greater effectiveness of silver nanoparticles on staphylococcus aureus. This study aimed to evaluate the antimicrobial effects of silver nanoparticles (NP) on air flora and water flora. We observed that the AgNps obtained were effective against air flora and water flora. These results suggest that AgNps synthesized may be used as antimicrobials in the waste water treatment and to control bioaerosols.