Chiral organic sulfoxides are important synthons and chiral auxiliaries in synthetic organic chemistry. Therefore synthesis of chiral sulfoxide is an active area of continuing research interest. The most common method for the preparation of sulfoxide is by the oxidation of their corresponding sulfides. Both the chemical and biological catalysts have been developed for this purpose. Though the reaction conditions for the preparation of chiral organic sulfoxides. With the hope that some of them will produce the desired sulfoxidation in a good enantiomeric excess. Being involving biological catalysts are milder and eco-friendly in comparison to those involving chemical catalysts, they are of limited practical use. This communication reports a crude preparation of chloroperoxidase from a plant which can be conveniently prepared and used for the transformation of different sulfides to its sulfoxide. This is the report of sulfoxide formation using a plant enzyme. The method for the preparation of enzymes from plants has been developed.