A novel hydrazide derivative of ciprofloxacin was synthesized and evaluated for its antibacterial activity against E. coli, B. subtilis, and P. auregenosa. The compound was purified by column chromatography and spectrally elucidated using U.V. and FTIR techniques. The compound has shown activity at 10 μg/mL concentration against gram negative bacterial strains (Pseudomonas auregenosa and E. coli). A simple and economic U.V. spectroscopic method was developed for the estimation of ciprofloxacin hydrazide. The derivative has shown absorption maximum at 266 nm. The developed method was validated for various parameters like ruggedness, robustness, accuracy, LOD and LOQ. The regression coefficient for the linearity was found to be 0.999. The linearity concentration range for the derivative was found to be 10 - 50 μg/mL. The present work raised a scope of further work on structural features of nucleus with biological activity.
