European Journal of Zoological Research
Abstract
Author(s): Annasamudram Saradamba, Pranav R. Buch, Hiral A. Murawala, Suresh Balakrishnan
Tyrosine Kinase receptors (RTKs) are known to play a role in the regulation of number of important cellular
activities. Growth factor signaling involves RTKs and mediates the proliferation of cells in various systems, making
these receptors attractive targets for checking tumorous growth. The fibroblast growth factor (FGF) receptors are
one such group of target receptors. Indolinone inhibitors such as SU5402, which bind irreversibly to these
receptors, are being widely used to block FGF signaling and thereby curb uncontrolled proliferation. In the current
study, we have used the regenerating fin of teleost fish Poecilialatipinna and studied the effect of SU5402 on this
non-cancerous model of extensive cell proliferation. Our results indicate an adverse effect of SU5402 on
regenerative outgrowth of fins and thereby reaffirm the anti-proliferative effect of this FGF receptor inhibitor.
Morphometric, immunohistochemical, biochemical and histological observations all explicitly show a slowdown in
the process of re-growth of amputated fins due to SU5402 treatment. The study also reveals a crucial role of FGF
signaling in successful fin regeneration.
