European Journal of Zoological Research
Abstract
Author(s): Forutan Salehi Nezhad, Abolghasem Lavvaf and Akram Yar Ahmadi
In order to determine how heat stress affects Sertoli cells and study the type of caused damages in these cells
affected by these conditions , this study aims at evaluating the response indicators of oxidative and the amount of
DNA damage by using the TUNEL method in Sertoli cells affected by heat stress at 42Ã?â??Ã?°C . Testes of 9 lambs of 3 to
10months provided in slaughters and then isolation and culture of sertoli cells was performed on these
samples.DNA damage and amount of reactive oxidative were studied in control groups under mild heat stress and
extreme heat stress by using the experimental Random design and statistical test (one way ANONA ) in a statistical
software SPSS. the Results of present study showed that by increasing temperature , the amount of living cells
decreases and the difference between the percentage of living cells and cells of the control group at 32 Ã?â??Ã?°C with
39Ã?â??Ã?°C groups and 42 Ã?â??Ã?°C groups is significant (P < 0.05 ) . Assessing by TUNEL staining showed that applying the
heat stress caused an increase in the number of damaged cells from DNA and this increase was significant in 42 Ã?â??Ã?°C
group compared with control group.
