This experiment was conducted to study the morphological features and essential oils content in two Satureja khuzistanica Jamzad populations in Tehran ecological conditions. First, seeds of two populations from the species Satureja khuzistanica Jamzad were collected from Lorestan Province, Iran. Collected seeds were evaluated to be from the correct genus and species by the help of experts in the Iranian Research Institute of forests and Rangelands. Seeds were planted in proper transplanting trays and at 8-10 leaves stage, healthy plants were transplanted in special pots and finally were moved to the main field. Plants were regularly monitored during the growing period. At full flowering stage, flowering shoots were harvested to measure their essential oil content. To do this, samples were dried under shadow in proper temperature. In laboratory, their essential oils were extracted by the method of water distillation. Results indicated that S. khuzistanica 14 had better morphological features such as plant height, branches length, stem length, leaf length / width ratio and canopy diameter than S. khuzistanica 13. However, stem length, the number of leaves, the number of branches and leaf length were higher in S. khuzistanica 13. Finally, S. khuzistanica 13 had higher shoot dry weight and essential oil content that resulted in higher essential oils yield.