Sparfloxacin ointments were prepared by fusion method. Simple, paraffin and emulsifying ointment base with two different permeation enhancers PEG 400 and Tween 80 were used for the study. In vitro drug release was studied through rat skin by using Franz-type diffusion cell. Antibacterial activity was carried out by filter paper disc method to find out the drug release of the ointment preparations. It was found that the zone of inhibition was higher for the drug with PEG 400 as base than other ointment preparations. It was concluded based on the results that the formulation having permeation enhancers in the formulation increases drug release
