The increasing pace of industrialization in public and private sectors along with urbanization, population explosion and green revolution are reflected in varying degree of water, soil and air pollution. Environmental pollution has a great health hazard to human, animals and plants with local, regional and global implications. Sugar industry plays an important role in the rural economy of India by uplifting the livelihood and creation of employment. The byproducts of sugar industry are also used as raw materials in different industries. However sugar industries posed a great environmental impact upon the surrounding environment as it releases various pollutants including huge amount of effluent. In the present investigation, laboratory experiments were conducted to determine the comparative phytotoxic effects of untreated and bioremediated sugar industry effluent with immobilized Spirogyra sp. The present research work includes the effects of different concentrations viz., 25%, 50%, 75% and 100% of sugar industry wastewater and bioremediated wastewater on percentage of seed germination, shoot length, root length, and some biochemical parameters viz., chlorophyll, protein, total carbohydrates, starch and free amino acids of Vigna radiata. The results of this research work revealed that, using undiluted effluent no seed germination was observed even after 7 days while in 75% diluted effluent 90% seeds were germinated after 7 days. While in bioremediated waste water, 100% seed germination was observed only after 3 days. The shoot length, root length and biochemical parameters showed inhibitory effects with increasing concentration of effluent. In case of bioremediated wastewater, strikingly most of the parameters showed stimulatory effects as compared to control.