Labeo rohita is fresh water teleost shows variation in the reproductive cycle. The entire cycle is divided into five phases- resting, preparatory, prespawning, spawning and postspawaning. Sertoli cells were observed at the periphery of primary spermatogonia. It consists of both dark and light stained nuclei. They were in large number during the early period of reproductive cycle which later decrease gradually upto spawning phase. The groups of interstitial cells were located in between the seminiferous lobules. It shows morphological variation throughout the cycle. In resting phase, they are elongated and rounded in shape with darkly stained nuclei. However they become thin, elongated aster like in the spawning phase. In the Postspawning phase, the spermatozoa degenerates and the primary spermatogonia start appear. The spermatogonia are being surrounded by sertoli cells. The interstitial cells are present around the seminiferous lobules.
