In this study the effect of different concentrations of pix as plant growth regulators include 0 (control), 0.5 ,1, 1.5 , 2 L.ha-1 on soluble sugars proline, phenolic compounds content and antioxidant enzymes activity such as catalase, peroxidase and poly phenol oxidase in leaf and root of cotton plant (Gossypium hirsutum L. cv Ci-Ocra) in vegetative phase mid under pots condition were evaluated. The result showed that pix spray on cotton shoot increased amount of soluble sugars in leaf. Also pix reduced proline content in root while was not affected amount of phenolic compounds in cotton plant. Our data showed pix application in different levels had not significant effect on catalse and peroxidase activity in leaf while decreased catalase activity and increased peroxidase activity in root than control. Different treatments of pix did not change poly phenol oxidase activity in root and leaf of cotton significantly.
