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Study of Musa DHN-1 Gene Expression from Banana Cultivar of Dwarf Cavendish under Salinity Treatment | Abstract
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Abstract

Study of Musa DHN-1 Gene Expression from Banana Cultivar of Dwarf Cavendish under Salinity Treatment

Author(s): Mehrdad Mahmoudi Souran, Gol Mohammad Dorrazehi, Mohammad Hossein Sangtrash, Hosseing Piry, Alireza Ainali, Abdolvahed Ghorbani Dadkaniand Mansour Dehvari

Soil salinity is an increasing problem, which causes stress response in plants and ultimately prevents the full expression of the genetic potential of the plants. In response to stressing factors, there are various genes with positive regulation, which can reduce the effects of stress and lead to adaptation and tolerance of plants. Dehydrins are proteins that affected by water stress, low temperature, salinity and abscisic acid (ABA) and they accumulate in vegetative organs, which suggests a protective role of this group of proteins in terms of water restrictions. Because of shallow roots and constant green covering crest, bananais very sensitive to the conditions that lead to lack of water. Banana meristem cultivation for is applicable in banana cultivation and transformation agendas. In this study, to investigate the MusaDHN-1 gene expression under salt stress conditions, apical meristems of banana, Dwarf Cavendish, were cultured in MS environment and after 4 months, Samples were transferred to pots for applying stress, and for 10 days with concentration of 0mM (control), 25mM, 50mM, 100mM, 150mM, NaCl were irrigated. RNA was extracted from leaf tissue samples, and the level of MusaDHN-1 gene expression was examined by RT-PCR method. The results showed high gene expression of MusaDHN-1 in terms of phenotypic and genotypic in conditions of salinity stress is involved in the plant tolerance and consistency. Studying the expression level of MusaDHN-1 gene is not the solution individually, but identifying a set of the co-expression genes and pattern study of their collective changes in response to different levels of stress are of particularly importance and also monitoring MusaDHN-1 gene expression level in other tissues such as roots, stems, and the impact of it on ions and osmolytes such as proline, could gives a better understanding of responses to stress conditions.