The present study was undertaken for assessing the level of heavy metals such as copper, nickel and zinc in water, water hyacinth and soil samples of Rupahi beel, Nagaon, Assam. Metals were detected using flame atomic absorption spectrophotometry. The results revealed that by and large all the three metals are present in all the samples. The average concentration of Cu, Ni and Zn in both water and soil of the beel are in the order Ni < Cu < Zn. However, the concentration in water hyacinth (both in root and leaf) is in the order Cu < Ni < Zn. The metal concentration in water hyacinth is much more than both in water and soil that signifies the absorption capacity of it.