An experiment was conducted to evaluate the efficacy of leaf extract of five medicinal weed plant on growth and sporulation of nematophagous fungus Paecilomyces lilacinus.The effect of leaf extract was assessed at the different time of interval such as 24hours, 48hours, 72hours, 96hours and 120hours respectively. After 120hours, the maximum mycelial growth was observed in Amaranthus spinosus (8.83cm), minimum in Lantana camara (7.16cm).The maximum number of spores/cm2 was observed in Veronica anagallis aquatica (5.9x106 ) and minimum in Gnaphalium perpureum (3.7x106 ).