In this study, the effects of hydro alcoholic extract of plantago major in rats were examined on the level of anxiety. In this experimental study, 30 male Wistar rats were used. Animals in groups of six rats including the control group received normal saline, group receiving epinephrine 1 mg/kg, groups receiving different doses of the extract (100 and 200 mg/kg), the group receiving pretreatment with epinephrine 1 mg/kg and then 100 mg/kg of extract. Elevated Plus-Maze test was used to assess anxiety. The results showed that plantago leaf extract at doses of 100 and 200 mg/kg significantly (p<0.05) created the anxiolytic effects and epinephrine prevented the anxiolytic effects of plantago. According to the results of this study, it seems that flavonoid compounds in plantago major leaf extract such as apigenin and its interaction with the adrenergic system is able to create anxiolytic effects
