An investigation has been made about the vesicular arbuscular mycorrhizal fungi colonization on some medicinal plants. A total number of ten medicinal plants species selected for the study . The predominant VAM spores observed in the soil sample includes Glomus and Gigaspora species. Among the rhizosphere soil examined, a maximum spore count of 80 spores/25 gram soil was reported in Brahmi (Bacopa monneiri) and a lowest of 15 spores/25 gram soil in Neela Amari (Indigofera tinctora). This study reveals the diversity of VAM of temperate medicinal plants.