In order to consider impact of biological and chemical fertilizers (N, P) on chlorophyll contentnt which exist in Artemisia annua L. an experiment was carried out in factorial design in completely randomized design with 4 replications in Zabol University in 2011. Treatments included chemical fertilizers (N, P) in 4 levels (N0P0, N40P40, N80P40, N80P80) and biological fertilizers in 4 levels (control, Nitroxin [include bacteria which stimulus growth (Azotobacter and Azospirillum)], Bio-phosphorus [(include bacteria which stimulus growth (Bacillus and Pseudomonas)] and Vemricompost fertilizer. About chlorophyll content, applying biological fertilizers specially Vermicompost has better impact that chemical fertilizers and Vermicompost + N80P80 had better impact on chlorophyll content in comparison with other experimental treatments.